The Truckee River runs through town, as does the Union Pacific Railroad, Interstate 80 (east-west) and US 395 (north-south).Reno has an extensive bus system called Citifare, which is provided by the Regional Transportation System of Washoe County. The bus system has its main terminal in downtown Reno and secondary terminals in Sparks and at Meadowood Mall in south Reno. The RTC also has a service Called Citilift that provides transport for handicapped people. PRIDE buses link Reno to Carson City.

The city also provides a free bus service up and down Virginia Street called the Sierra Spirit, whose bright yellow busses are easily recognizible. This regular service is free of charge.

Amtrak also serves the city of Reno. Amtrak train 5, the westbound California Zephyr, departs Reno every morning and serves the cities of Truckee, Colfax, Roseville, Sacramento, Davis, Martinez, Emeryville, and Sacramento. Amtrak train 6, the eastbound California Zephyr, departs Reno every afternoon and serves the cities of Sparks, Winnemucca, Elko, Salt Lake, Provo, Helper, Green River, Grand Junction, Glenwood Springs, Denver, Omaha, Galesburg, and Chicago.

Amtrak California Thruway Motorcoaches also arrive and depart Reno four times daily in each direction connecting to and from the Coast Starlight, Capitol Corridor, and San Joaquin trains at Sacramento, California. The city is served by Reno/Tahoe International Airport, with general aviation traffic also handled by Reno Stead Airport.